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Title: In-Vitro Antioxidant Activity of Endophytic Fungi Isolated from Medicinal Plants
Authors: Palak, Middha
Vasudhara, M. (Guide)
Keywords: Endophytes;Free Radicals;Secondary Metabolites;Tinospora Cordifolia;Terminalia Arjuna
Issue Date: 13-Sep-2017
Abstract: Endophytic fungi are microorganisms living inside plants are a rich source of novel metabolic compounds. The aim of this project was to isolate the endophytic fungi from the stem of Tinospora cordifolia and the bark of Terminalia arjuna, screening for antioxidant activity of the crude fungal extracts obtained from the endophytic fungi and fractionation of these fungal extracts along with antioxidant assay of eluted fractions. Antioxidant activity of methanolic fungal extracts was evaluated by DPPH free radical scavenging assay. A total of 11 endophytic fungi were isolated from Tinospora cordifolia and 5 from Terminalia arjuna. All the obtained fungal extracts were investigated for antioxidant activity from which 5 isolated fungal extracts from Tinospora cordifolia, 1 from Terminalia arjuna and 2 from Xylaria species showed positive results for antioxidant activity. G1 and G3 fungal extracts obtained from Tinospora cordifolia showed significant scavenging activity with the value of 40% and 45% at a concentration of 1000µg/ml. The fungal extract ARJ1 from Terminalia arjuna showed 55% scavenging activity at the concentration of 1000µg/ml. XF-2 and XF-4 from Xylaria sp. showed maximum antioxidant activity. XF-2 was observed to have 71% antioxidant activity even at a lower concentration of fungal extracts. As the concentration of fungal extract increased the scavenging activity also increased significantly for all the fungal extracts except XF-2 as it did not show a much significant increase in the scavenging activity with the varying concentration. The preparative TLC of 2 fungal extracts from Tinospora cordifolia and Terminalia arjuna was done to separate the fractions. All Fractions were screened for antioxidant activity, only two fractions D and F from Tinospora cordifolia and b and c from Terminalia arjuna showed positive results for antioxidant activity. Phytochemical analysis was also done. Endophytic fungi were isolated from the medicinal plants Tinospora cordifolia and Terminalia arjuna. The phenolics and flavonoids were present in the fungal extract could be the reason for the antioxidant activity. Hence the above results indicate that endophytic fungus obtained from above-mentioned source can be exploited as a potential source of antioxidants.
Description: Master of Science -Biotechnology
Appears in Collections:Masters Theses@SCBC

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